EcoRII: a restriction enzyme evolving recombination functions?
نویسندگان
چکیده
منابع مشابه
Reliable detection of DNA cytosine methylation at CpNpG sites using the engineered restriction enzyme EcoRII-C.
Methylation of cytosine to 5-methylcytosine (mC) is the most important epigenetic DNA alteration in eukaryotes. Cytosine methylation is involved in establishing a silenced chromatin stage through interaction with DNA-binding proteins and recruitment of histone deacetylases and other histone-modifying enzymes leading to chromatin remodeling. In this fashion, DNA methylation is connected with pro...
متن کاملRestriction Enzyme Recognition Sequence
A critical and difficult part of characterizing restriction enzymes and methylases is the identification of recognition sequences. To simplify this process, we have developed a plasmid transformation method along with a computer program named RM search that determines the exact recognition sequences for given restriction and modifica-
متن کاملPathogenicity Study and Restriction Enzyme Profile of a Recently Isolated Infectious Bursal Disease Virus in Iran
The pathogenicity of a recent isolate of Infectious bursal disease virus (IBDV), IR499, isolated from a nonvaccinated flock with 17.5 % mortality rate in susceptible SPF chickens, chickens embryos and broilers was discussed. The molecular characterization of the virus based on the restriction fragment length polymorphism (RFLP) pattern was also investigated. The mortality rates were 85% and 22%...
متن کاملRFLP Analysis of Beta‐Lactoglobulin Gene in Swamp and Murrah Buffaloes Using a Single Restriction Enzyme
An attempt has been made to analyze the distribution of the beta-lactoglobulin genotype in swamp buffaloes and Murrah buffaloes utilizing polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Blood samples were taken from 50 swamp buffaloes and 50 Murrah buffaloes. The DNA was extracted by the phenol-chloroform method. The PCR-RFLP was performed using beta-lactoglobulin ...
متن کاملAsymmetric photocross-linking pattern of restriction endonuclease EcoRII to the DNA recognition sequence.
The EcoRII homodimer engages two of its recognition sequences (5'-CCWGG) simultaneously and is therefore a type IIE restriction endonuclease. To identify the amino acids of EcoRII that interact specifically with the recognition sequence, we photocross-linked EcoRII with oligonucleotide substrates that contained only one recognition sequence for EcoRII. In this recognition sequence, we substitut...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2002
ISSN: 0261-4189,1460-2075
DOI: 10.1093/emboj/cdf514